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In vitro analysis of the proliferation potential and colony forming efficiency of stem cells isolated from dental pulp (DPSC) and apical papilla (SCAP), cultured in standard and pro-mineralizing conditions

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Ewa Chomik, Paulina Bautembach-Koberda, Katarzyna Schütz, Tadeusz Pawełczyk, Izabela Maciejewska



2/2013/XLI s. 15–24
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Fraza do cytowania: Chomik E., Bautembach-Koberda P., Schütz K., Pawełczyk T., Maciejewska I. In vitro analysis of the proliferation potential and colony forming efficiency of stem cells isolated from dental pulp (DPSC) and apical papilla (SCAP), cultured in standard and pro-mineralizing conditions. Dental Forum. 2013;XLI(2):15–24.

Introduction. Recent data point to unerupted third molars as a promising source of stem cells (SC). Tooth derived SC are clonogenic and present a capacity for self-renewal and colony formation. Additionally, an environmental stimulation induces an in vitro differentiation of SC into multiple lineages, including odontoblasts. Aim of the study. The aim of the study was to evaluate the in vitro potential for proliferation and colony formation by stem cells derived from both dental pulp and apical papilla cultured in both standard medium (control and primary group) and medium modified with ingredients that stimulate mineralization (experimental group). Material and methods. Right after odontectomy the dental pulp and apical papilla were digested with dispase and collagenase type I. DPSCs and SCAPs were sorted using anti STRO-1, CD146, CD34, CD45 antibodies by means of the MACS method. Thereafter, the cells from the initial and control groups were cultured in a standard medium. The medium of the experimental group was additionally modified with ingredients that stimulated mineralization. To assess the cells commitment, the rate of proliferation and colony formation were examined. Results. The analysis showed that SCAPs from all the examined groups proliferated faster and formed more numerous and larger colonies compared to DPSCs. Environmental stimulation reduced proliferation and the ability to form colonies in both the DPSCs and SCAPs lineages. Conclusion. Faster proliferation and a higher ability to form colonies indicates the lower commitment of SCAPs compared to DPSCs. Additionally, the slower proliferation of stem cells from the experimental group suggests their more advanced commitment and differentiation. Although the SCAPs and DPSCs present different degrees of maturation, both cell lineages seem to be promising sources of stem cells.

Key words: stem cells, dental pulp, DPSC, apical papilla, SCAP.





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